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The running protocol was as follows: 94°C for 3 min, followed by 40 cycles at 94°C for 30 s; 58°C for 30 s; 72°C for 30 s; and a final extension of 72°C for 5 min. The amplification of HvActin (Accession number HORVU1Hr1G002840) was employed as an internal standard. All the reactions were run in triplicate.
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Full Article. Figures & data. References. Citations. Metrics. Reprints & Permissions. View PDF. Two component high affinity nitrate transport system, NAR2/NRT2, has been …
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NRT2 family of nitrate transporters normally require a partner protein, NAR2 (nitrate assimilation related protein), to transport nitrate to a high-affinity (high affinity nitrate transport system, HATS), although its role is still not well understood. In this study, the CcNRT2.1 and CcNAR2 cDNAs from of Capsicum chinense were cloned and …
Immunochemical analysis showed that both HVNRT2 and HvNAR2 proteins were co-localized in the plasma membrane of the roots, and an affinity column binding analysis using recombinant proteins suggests that the C-terminus of Hv nRT2.1 is possibly involved in its binding to the Hv NAR2.3 central region. A high affinity transport system …
Abstract. The NAR2 protein of Chlamydomonas reinhardtii has no known transport activity yet it is required for high-affinity nitrate uptake. Arabidopsis (Arabidopsisthaliana) possesses two genes, AtNRT3.1 and AtNRT3.2, that are similar to the C. reinhardtiiNAR2 gene.AtNRT3.1 accounts for greater than 99% of NRT3 mRNA and is …
However, the NAR2 motifs that interact with NRT2s for their plasma membrane (PM) localization and nitrate transporter activity have not been functionally characterized. In this study, OsNAR2.1 mutations with different carbon (C)-terminal deletions and nine different point mutations in the conserved regions of NAR2 homologs in plants …
The uptake of nitrate (NO 3 −) by roots cells from the soil solution is the main process of nitrogen (N) acquisition in most herbaceous plant species (Marschner, 1995).It relies on the combined activity of both high- and low-affinity transport systems (HATS and LATS, respectively) that together ensure an efficient intake over a wide range of external …
Function of NAR2 as a Nitrate Accessory Protein. The plant NAR2-type genes were first named WR3 identified by expression that was induced by wounding 1 and pathogens. 2 The involvement of NAR2 in the high-affinity transport system (HATS) for nitrate was initially identified genetically using mutants in Chlamydomonas reinhardtii, in …
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The stronger NAR2 adsorption as compared to NAR3 (cf., Table 3, column 3) qualitatively agrees with results obtained theoretically by the DFT method. This can be attributed to the stronger electron donating effect of the O-Benzoyl groups in NAR2, which promotes stronger adsorption on Fe (110), as compared to the O–Acetic groups in NAR3.
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Two component high affinity nitrate transport system, NAR2/NRT2, has been defined in several plant species. In Arabidopsis, AtNAR2.1 has a role in the targeting of AtNRT2.1 to the plasma membrane. The gene knock out mutant atnar2.1 lacks inducible high-affinity transport system (IHATS) activity, it also shows the same inhibition of lateral root ...
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Root cell membrane potentials in R1, R2 and R3 regions, before (0 h) and after (4 and 24 h) NO 3-exposure were recorded. At 0 h, after the stabilization of E m (5 min) in a basic buffer solution without NO 3-, the E m values were significantly different among the three root regions (Fig. 1).In particular, R1 showed a higher membrane potential …
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Screening using the membrane yeast two-hybrid system and Xenopus oocytes for nitrogen-15 (15 N) uptake demonstrated that either R100G or D109N point mutations impaired the OsNAR2.1 interaction with OsNRT2.3a. Western blotting and visualization using green fluorescent protein fused to either the N- or C-terminus of …
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Construction of Vectors and Rice Transformation. The open reading frames (ORF) of OsNAR2.1 (AP004023) and OsNRT2.3a (AK109776) were amplified from the full-length cDNA of rice cv. Nipponbare, and the primer sequences were shown in Supplementary Table S1.PrimeSTAR HS DNA Polymerase (TaKaRa Biotechnology Co., …
The current study was conducted to demonstrate the possible roles of exogenously applied flavonoid naringenin (Nar) on the efficiency of PSII photochemistry and the responses of chloroplastic antioxidant of salt and osmotic-stressed Phaseolus vulgaris (cv. Yunus90). For this aim, plants were grown in a hydroponic culture and were treated …
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Methylophaga nitratireducenticrescens JAM1 is the only reported Methylophaga species capable of growing under anaerobic conditions with nitrate as electron acceptor. Its genome encodes a truncated denitrification pathway, which includes two nitrate reductases, Nar1 and Nar2; two nitric oxide reductases, Nor1 and Nor2; and …